Python RPI : How do i dynamically adjust the volume of my wav file currently playing

Currently using Raspberry Pi 3, pyaudio , ReSpeaker 2-Mics Pi HAT

How do I adjust the volume of the .wav file playing using python in RPI? ( the output is through respeaker 3.5mm audio jack connected by an earpiece)

I need the RPI to play and adjust the volume dynamically without needing me to change it manually

Not accessing the alsamixer using the command in command prompt. Need some advice on how to proceed.

Below is the current progress of my code ( this code will be in a while loop playing ) :

            filename ="output.wav"
            
            RESPEAKER_RATE = 16000
            RESPEAKER_CHANNELS = 2
            RESPEAKER_WIDTH = 2
            RESPEAKER_INDEX = 0  
            chunk = 1024
            wf = wave.open(filename, 'rb')
            p = pyaudio.PyAudio()
            stream = p.open(
                    format = p.get_format_from_width(RESPEAKER_WIDTH),
                    channels = RESPEAKER_CHANNELS,
                    rate = RESPEAKER_RATE,
                    output = True
                )
            
           
            
            data = wf.readframes(chunk)
            while data != '':
                stream.write(data)
                data = wf.readframes(chunk)
            
            stream.stop_stream()
            stream.close()
            p.terminate()

Tried this code but not really what I am trying to achieve

Trying to change it whilst it is playing rather than changing it before

from pydub import AudioSegment

song = AudioSegment.from_wav("output.wav")
song = song - 60
quieter_song_data = song.get_array_of_samples()
quieter_song_fs = song.frame_rate4

from Python RPI : How do i dynamically adjust the volume of my wav file currently playing