Javascript - File upload; check if image has transparent background

I want to check the image transparency and display an error message if the image background is not transparent. I have the function hasAlpha(file) to check it the file has a transparent background but I am not sure how to pass it through the function uploadFile(file) function which already checks for file size.

I think I can duplicate the file size if statement inside of if (xhr.upload) {} and check if the uploaded image is transparent but I am not sure how to incorporate the function hasAlpha(file) with the rest of the code.

// File Upload
//
function ekUpload() {
  function Init() {
    console.log("Upload Initialised");

    var fileSelect = document.getElementById("file-upload"),
      fileDrag = document.getElementById("file-drag"),
      submitButton = document.getElementById("submit-button");

    fileSelect.addEventListener("change", fileSelectHandler, false);

    // Is XHR2 available?
    var xhr = new XMLHttpRequest();
    if (xhr.upload) {
      // File Drop
      fileDrag.addEventListener("dragover", fileDragHover, false);
      fileDrag.addEventListener("dragleave", fileDragHover, false);
      fileDrag.addEventListener("drop", fileSelectHandler, false);
    }
  }

  function fileDragHover(e) {
    var fileDrag = document.getElementById("file-drag");

    e.stopPropagation();
    e.preventDefault();

    fileDrag.className =
      e.type === "dragover" ? "hover" : "modal-body file-upload";
  }

  function fileSelectHandler(e) {
    // Fetch FileList object
    var files = e.target.files || e.dataTransfer.files;

    // Cancel event and hover styling
    fileDragHover(e);

    // Process all File objects
    for (var i = 0, f;
      (f = files[i]); i++) {
      parseFile(f);
      uploadFile(f);
    }
  }

  // Output
  function output(msg) {
    // Response
    var m = document.getElementById("messages");
    m.innerHTML = msg;
  }

  function hasAlpha(file) {
    var canvas = file.getElementById("file-image");
    var ctx = canvas.getContext("2d");
    var data = file.getImageData(0, 0, canvas.width, canvas.height).data,
      hasAlphaPixels = false;
    for (var i = 3, n = data.length; i < n; i += 4) {
      if (data[i] < 255) {
        hasAlphaPixels = true;
        break;
      }
    }
    return hasAlphaPixels;
  }

  function parseFile(file) {
    console.log(file.name);
    output("<strong>" + encodeURI(file.name) + "</strong>");

    // var fileType = file.type;
    // console.log(fileType);
    var imageName = file.name;

    var isGood = /\.(?=svg|jpg|png|jpeg)/gi.test(imageName);
    if (isGood) {
      document.getElementById("start").classList.add("hidden");
      document.getElementById("response").classList.remove("hidden");
      document.getElementById("notimage").classList.add("hidden");
      // Thumbnail Preview
      document.getElementById("file-image").classList.remove("hidden");
      document.getElementById("file-image").src = URL.createObjectURL(file);
    } else {
      document.getElementById("file-image").classList.add("hidden");
      document.getElementById("notimage").classList.remove("hidden");
      document.getElementById("start").classList.remove("hidden");
      document.getElementById("response").classList.add("hidden");
      document.getElementById("file-upload-form").reset();
    }
  }

  function uploadFile(file) {
    var xhr = new XMLHttpRequest(),
      fileInput = document.getElementById("class-roster-file"),
      fileSizeLimit = 1024; // In MB

    if (xhr.upload) {
      // Check if file is less than x MB
      if (file.size <= fileSizeLimit * 1024 * 1024) {
        // File received / failed
        xhr.onreadystatechange = function(e) {
          if (xhr.readyState == 4) {
            // Everything is good!
            // document.location.reload(true);
          }
        };

        // Start upload
        xhr.open(
          "POST",
          document.getElementById("file-upload-form").action,
          true
        );
        xhr.setRequestHeader("X-File-Name", file.name);
        xhr.setRequestHeader("X-File-Size", file.size);
        xhr.setRequestHeader("Content-Type", "multipart/form-data");
        xhr.send(file);
      } else {
        output("Please upload a smaller file (< " + fileSizeLimit + " MB).");
      }
    }
  }

  // Check for the various File API support.
  if (window.File && window.FileList && window.FileReader) {
    Init();
  } else {
    document.getElementById("file-drag").style.display = "none";
  }
}
ekUpload();

form {
  display: flex;
  flex-direction: column;
  justify-content: center;
  align-items: left;
  border: 1px solid;
  cursor: pointer;
  height: 90px;
}

img {
  border: 1px solid;
  height: 60px;
  width: 60px;
}

.uploader input[type="file"],
.hidden {
  display: none;
}

<!-- Upload  -->
<form id="file-upload-form" class="uploader">
  <input id="file-upload" type="file" name="fileUpload" accept="image/*" />
  <label for="file-upload" id="file-drag">
    <img id="file-image" src="#" alt="Preview" class="hidden">
    <div id="start">
      <i class="fa fa-download" aria-hidden="true"></i>
      <div>Select a file or drag here</div>
      <div id="notimage" class="hidden">Please select an image</div>
    </div>
    <div id="response" class="hidden">
      <div id="messages"></div>
    </div>
  </label>
</form>
<div class="error hidden">Your logo must have a transparent background. Please set RBGa

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